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Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient

Paper ID Volume ID Publish Year Pages File Format Full-Text
390 40 2012 9 PDF Available
Title
Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient
Abstract

Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD.

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Keywords
Sickle cell disease; Red blood cell; Cell biomechanics; Cell membrane; Optical measurement
First Page Preview
Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 11, November 2012, Pages 4130–4138
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering