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Hepatocytes exhibit constant metabolic activity on carboxymethylcellulose-based hydrogel with high phenolic hydroxy group content

Paper ID Volume ID Publish Year Pages File Format Full-Text
3914 198 2010 6 PDF Available
Title
Hepatocytes exhibit constant metabolic activity on carboxymethylcellulose-based hydrogel with high phenolic hydroxy group content
Abstract

Maintenance of cellular number and functions of attached hepatocytes during culture is an important requirement for hepatocyte-based drug screening applications. In this study, two carboxymethylcellulose (CMC)-based hydrogels were used as hepatocyte culture substrata to improve the stability of cellular adhesiveness, bioactivity and hepatic function of primary rat hepatocytes. The hydrogels were prepared from aqueous solutions of CMCs with 16.7 (CMC-Ph16) and 7.3 (CMC-Ph7) phenolic hydroxy (Ph-OH) groups per 100 repeat units of uronic acid, via peroxidase-catalyzed crosslinking reaction between Ph-OH groups. Hepatocytes formed aggregates on both hydrogels but the aggregates on CMC-Ph16 gel had more flattened configuration than on CMC-Ph7 gel. The flat aggregates stably immobilized onto CMC-Ph16 gel while spherical aggregates on CMC-Ph7 gel could be easily detached from the surface. In addition, hepatocytes on CMC-Ph16 gel showed more stable mitochondrial dehydrogenase activity and albumin secretion function for 7 days than on CMC-Ph7 gel, tissue culture polystyrene (TCPS), and TCPS coated with collagen (CL). The retention of albumin secretion function per well after 7 days of seeding was 86% on CMC-Ph16 gel, 18% on CMC-Ph7 gel, 9% on TCPS, and 6% on CL. These results demonstrate that CMC-Ph16 gel is a good candidate substratum for hepatocyte-based drug testing applications.

Keywords
Carboxymethylcellulose with phenolic hydroxyl groups; Enzymatic gelation; Rat primary hepatocyte; Maintenance of function
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Hepatocytes exhibit constant metabolic activity on carboxymethylcellulose-based hydrogel with high phenolic hydroxy group content
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 51, Issue 3, 15 September 2010, Pages 147–152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us