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Zearalenone determination in corn silage samples using an immunosensor in a continuous-flow/stopped-flow systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
3925 199 2010 7 PDF Available
Title
Zearalenone determination in corn silage samples using an immunosensor in a continuous-flow/stopped-flow systems
Abstract

Zearalenone (ZEA) is a mycotoxin produced by a variety of Fusarium fungi that infects cereals. ZEA may accumulate in cereals before harvest time. This paper describes the development of an immunosensor coupled to glassy carbon electrode (GCE) modified with multiwall carbon nanotubes (MWCNT) (CNT-GCE) integrated with a continuous-flow systems for rapid and sensitive quantification of ZEA in corn silage samples. Mouse monoclonal anti-ZEA antibodies were immobilized on a rotating disk. The ZEA in corn sample is allowed to compete immunologically with ZEA bound to horseradish peroxidase (HRP) for the immobilized antibodies. HRP in presence of hydrogen peroxide (H2O2) catalyzes the oxidation of 4-tert-butylcatechol (4-TBC), whose back electrochemical reduction was detected on CNT-GCE at −0.15 V. The total assay time was 15 min. The electrochemical immunosensor showed higher sensitivity and lower detection limits than the standard ELISA method, which shows potential for detecting ZEA in foods and feeds diagnosis.

Keywords
Enzyme immunoassays; Zearalenone; Multiwall carbon nanotubes; Horseradish peroxidase; Flow injection analysis
First Page Preview
Zearalenone determination in corn silage samples using an immunosensor in a continuous-flow/stopped-flow systems
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 51, Issues 1–2, 15 August 2010, Pages 7–13
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering