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Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3928 199 2010 8 PDF Available
Title
Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system
Abstract

An aqueous two-phase system (ATPS) composed of a thermoseparating copolymer EO50PO50 [50% (w/w) ethylene oxide and 50% (w/w) propylene oxide] and K2HPO4 was used to extract lysozyme from hen egg white over four successive extractions, using the recovery and recycling of phase components. Extraction was performed in two stages. In the first stage the primary two-phase system of 40% EO50PO50/10% K2HPO4 (w/w) was formed and the lysozyme partitioned to the top copolymer-rich phase while the contaminant proteins remained in the bottom phosphate-rich phase. In the next stage the copolymer-rich phase was isolated and subjected to temperature-induced phase separation. The resultant secondary two-phase system was composed of a concentrated solution of copolymer and a water solution of lysozyme. Next, the concentrated solution of EO50PO50 recovered after thermoseparation was mixed with the phosphate solution recovered from the primary system and a new portion of hen egg white added to obtain a new lysozyme extraction system. The specific activity of lysozyme preparations obtained during the four successive extractions ranged from 38,438 to 42,907 U/mg of protein.

Keywords
Lysozyme; Egg white; Bioseparation; Aqueous two-phase systems; Temperature-induced phase separation; EOPO
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Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 51, Issues 1–2, 15 August 2010, Pages 24–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us