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Characterization of detergent stable and feather degrading serine proteases from Bacillus mojavensis A21

Paper ID Volume ID Publish Year Pages File Format Full-Text
3932 199 2010 11 PDF Available
Title
Characterization of detergent stable and feather degrading serine proteases from Bacillus mojavensis A21
Abstract

The present study describes the characterization of crude protease from Bacillus mojavensis A21 and its evaluation in detergent and chicken feathers hydrolysis. The strain was found to produce at least six major extracellular proteases as shown by casein-zymography. The optimum pH and temperature for proteolytic activity were 8.0–11.0 and 60 °C, respectively. The crude protease showed extreme stability towards non-ionic (5% Tween 80 and 5% Triton X-100) and anionic (1% SDS) surfactants, and relative stability towards oxidizing agents. Additionally, it showed excellent stability and compatibility with various solid (7 mg/ml) and liquid (1%; v/v) detergents at temperatures from 30 to 50 °C. In the presence of solid detergents, the enzyme preparation retained 100% of its initial activity after pre-incubation for 1 h at 40 °C with Axion and Ariel, followed by Nadhif (87%), Dixan (85%) and New Det (82%). With liquid detergents, the enzyme preparation retained 100% of its original activity after pre-incubation for 1 h at 40 °C with Dixan and Nadhif. Wash performance analysis revealed that A21 crude protease could effectively remove blood-stains. In addition, B. mojavensis A21 proteolytic preparation showed important feather degrading activity.Considering its promising properties, B. mojavensis A21 enzymatic preparation may be considered a potential candidate for future use in biotechnological processes, particularly in detergent and in the processing of poultry waste.

Keywords
Bacillus mojavensis; Alkaline serine proteases; Detergent stable; Keratinolytic activity
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Characterization of detergent stable and feather degrading serine proteases from Bacillus mojavensis A21
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 51, Issues 1–2, 15 August 2010, Pages 53–63
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us