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Cellulases, xylanases, β-glucosidase and ferulic acid esterase produced by Trichoderma and Aspergillus act synergistically in the hydrolysis of sugarcane bagasse

Paper ID Volume ID Publish Year Pages File Format Full-Text
3934 199 2010 7 PDF Available
Title
Cellulases, xylanases, β-glucosidase and ferulic acid esterase produced by Trichoderma and Aspergillus act synergistically in the hydrolysis of sugarcane bagasse
Abstract

Trichoderma reesei and Aspergillus awamori enzymes were concentrated, pooled and assessed for the hydrolysis of steam-pretreated sugarcane bagasse. The enzyme profile of T. reesei gave (IU/L): 1700 FPA, 20,000 CMCase, 340 β-glucosidase and 12,600 xylanase. FPA and CMCase activities that were 4-fold higher than those of A. awamori (420 and 4900 IU/L, respectively). However the β-glucosidase and xylanase activities were 134- and 6-fold lower than those of A. awamori (45,600 and 79,100 IU/L, respectively). Furthermore, A. awamori produced ferulic acid esterase (160 IU/L) which acts synergistically with cellulolytic–xylanolytic enzymes in the hydrolysis of lignocellulosic materials. The FPA and CMCase activities in the T. reesei–A. awamori blends were enhanced synergistically by 2-fold. Moreover, the hydrolytic effectiveness of the blends was superior to the use of unblended T. reesei or A. awamori enzymes, under corresponding conditions (10 FPU/g bagasse, 20 g bagasse/L and 50 °C). Hydrolysis experiments, presenting either 20 or 200 g/L bagasse, resulted in 3.9 or 40 g glucose/L, respectively. These values corresponded to 41% cellulose hydrolysis within 6 or 24 h, respectively. A. awamori enzymes hydrolyzed 91% (1.7 g/L xylose) of the residual xylan in the bagasse within 6 h in experiments presenting 20 g/L bagasse.

Keywords
Trichoderma reesei; Aspergillus awamori; Enzyme blends and synergy; Sugarcane bagasse hydrolysis; Cellulases; Xylanases; Ferulic acid esterase
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Cellulases, xylanases, β-glucosidase and ferulic acid esterase produced by Trichoderma and Aspergillus act synergistically in the hydrolysis of sugarcane bagasse
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 51, Issues 1–2, 15 August 2010, Pages 72–78
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us