fulltext.study @t Gmail

Magnetic polymeric nanospheres as an immobilized metal affinity chromatography (IMAC) support for catalase

Paper ID Volume ID Publish Year Pages File Format Full-Text
3941 200 2010 6 PDF Available
Title
Magnetic polymeric nanospheres as an immobilized metal affinity chromatography (IMAC) support for catalase
Abstract

Novel magnetic nanospheres with an average size of 118 nm utilizing N-methacryloyl-(l)-cysteine methyl ester (MAC) as a monomer were prepared by surfactant free emulsion polymerization of 2-hydroxyethyl methacrylate (HEMA) and MAC conducted in an aqueous dispersion medium. MAC was synthesized using methacryloyl chloride and l-cysteine methyl ester. l-Cysteine groups of the mag-poly(HEMA–MAC) nanospheres were chelated with Fe3+ ions. Specific surface area of the nonporous nanospheres was found to be 2452 m2/g. Mag-poly(HEMA–MAC)-Fe3+ nanospheres contained 0.81 mmol sulphur/g polymer were used in the adsorption of catalase in batch system. Using an optimized adsorption protocol, a very high loading of 820 mg catalase/g nanosphere was obtained. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The immobilized catalase has more resistence to temperature inactivation than that of their free form. The optimum pH value of catalase was not affected by the immobilization reaction, but the pH profile was broadened for the immobilized enzyme. Kinetic parameters were determined for immobilized catalase as well as for the free enzyme. The values of the Michaelis constant Km of catalase were significantly smaller (ca. 2.5 times) upon immobilization, indicating increased affinity of the enzyme for its substrate, whereas Vmax value of free catalase was higher than that of the immobilized enzyme. It was also observed that enzyme could be repeatedly adsorbed and desorbed on the mag-poly(HEMA–MAC)-Fe3+ nanospheres without loss of adsorption capacity or enzymatic activity.

Keywords
Catalase; Nanospheres; Support; IMAC; Cysteine
First Page Preview
Magnetic polymeric nanospheres as an immobilized metal affinity chromatography (IMAC) support for catalase
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 2, 15 April 2010, Pages 159–164
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us