fulltext.study @t Gmail

Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger

Paper ID Volume ID Publish Year Pages File Format Full-Text
3949 200 2010 8 PDF Available
Title
Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger
Abstract

A simple lumped kinetic model was used to simulate the single- and two-component breakthrough curves for the cation-exchange adsorption of pure α-lactalbumin (ALA) and β-lactoglobulin (BLG) onto a 5-ml SP Sepharose FF column at pH 3.7 and flow rate of 2 ml min−1. When compared to equivalent experimental results, the model accurately predicted the single-component BLG adsorption profiles using Langmuir isotherm parameters (qm, maximum binding capacity of the adsorbent; Kd, dissociation constant for the protein–adsorbent interaction) obtained in batch experiments. The breakthrough curve for ALA, however, was well predicted only after increasing qm which indicates the occurrence of additional adsorption processes in the packed-bed relative to the batch system. An overshoot of the concentration of BLG in the bed exit stream observed experimentally in the two-component system, was only predicted after correcting the two isotherm parameters in order to account for the unexpected finding that the weakly bound ALA was able to displace the strongly bound BLG. A fitting mechanism was proposed for this situation. The correction factors employed for the pure binary mixture were used to simulate the breakthrough curves of the two proteins in experiments conducted with whey concentrate in each of the two stages of a novel separation process, and there was agreement between the experimental and theoretical results. These considerations should be helpful in developing a model compatible with the proposed mechanisms of adsorption for these two proteins.

Keywords
Simulation; Simple kinetic model; α-Lactalbumin; β-Lactoglobulin; Whey concentrate; Packed-bed adsorption
First Page Preview
Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 2, 15 April 2010, Pages 221–228
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us