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Improving Jatropha curcas seed protein recovery by using counter current multistage extraction

Paper ID Volume ID Publish Year Pages File Format Full-Text
3961 201 2010 8 PDF Available
Title
Improving Jatropha curcas seed protein recovery by using counter current multistage extraction
Abstract

Jatropha curcas seed press cake contains 23 wt% proteins (dry basis). Due to the toxic compounds in Jatropha, we will use the proteins for non-food applications. Related to non-food applications, an efficient protein extraction to obtain a high protein recovery and high protein concentration with good protein functional properties is required. To achieve this, we conducted protein extractions at room temperature by using different solvents at various solvent to solid ratios and performed multistage counter current extraction to improve protein recovery and protein concentration. We obtained the highest protein recovery of 82% by using 0.055 M NaOH at solvent to solid ratio of 10 g/g after four-stage counter current extraction. By using 0.055 M NaOH extraction at lower solvent to solid ratio of 4 g/g, protein recovery was 35% after one-stage extraction and improved up to 71% with a high protein concentration of 63 mg/mL after four-stage counter current extraction. The highest amount of protein was precipitated from crude extracts at pH 4–5.5 and resulted on precipitate with protein content of 70%. The multistage counter current extraction did not influence Jatropha seed protein molecular weight distributions, which were within the range of 3–98 kDa.

Keywords
Jatropha curcas; Counter current extraction; Protein recovery; Molecular weight distribution; Precipitation; Biorefinery
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Improving Jatropha curcas seed protein recovery by using counter current multistage extraction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issues 1–2, 15 June 2010, Pages 16–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us