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Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
3963 201 2010 8 PDF Available
Title
Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides
Abstract

Continuous galacto-oligosaccharides (GOS) synthesis by bead-immobilized β-galactosidase and nanofiltration fractionation of derived sugar mixtures by cellulose acetate membranes are investigated. Upon enzyme adsorption assays (8 mL/g of solution/resin ratio, 150 mg/mL of initial enzyme concentration and 14 ± 1.4 mg protein/g resin), a Langmuir-type isotherm is observed. Enzyme concentration of 10 U/mL, selected in batch reactor experiments, is used for continuous GOS production carried out at lactose concentrations of 150 g/L and 300 g/L, and at 40 °C. Total dissolved solids concentration influences both reaction and saccharide mixtures fractionation. All observed sugar retention coefficients, mainly for monosaccharides, increase with transmembrane pressure and a gap between the retention values of synthesized disaccharides and lactose (87% versus 77%, respectively, for 2 bar/40 °C) is displayed as a function of the effective transmembrane pressure and total sugars concentration. At 150 g/L total sugars in feed, trisaccharides are totally retained. Doubling sugars concentration, trisaccharides retentions decreased for all applied pressures, reaching 90%.

Keywords
Nanofiltration; Enzyme immobilization; Oligosaccharides synthesis; Saccharides fractionation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issues 1–2, 15 June 2010, Pages 29–36
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us