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Design and performance evaluation of a microwave based low carbon yielding extraction technique for naturally occurring bioactive triterpenoid: Oleanolic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
3967 201 2010 8 PDF Available
Title
Design and performance evaluation of a microwave based low carbon yielding extraction technique for naturally occurring bioactive triterpenoid: Oleanolic acid
Abstract

An ecofriendly microwave-assisted extraction (MAE) technique with a low carbon output was developed for the rapid extraction of bioactive oleanolic acid from Gymnema sylvestre. Several different influential extraction parameters such as microwave power, extraction time, solvent type, solvent composition, preleaching time, loading ratio and extraction cycle were studied in a systematic fashion for the determination of optimum extraction conditions. Under optimum conditions, 8 min of MAE produced a maximum yield of 7.6% (w/w) of oleanolic acid which was found to be 4 times, 3 times and 1.2 times more efficient than maceration, stirring extraction and heat reflux extraction, respectively. Extracts obtained from 8 min of MAE showed better antioxidant activity when compared to other conventional methods. No degradation of the target analyte was observed at the optimum conditions as evidenced from the stability studies performed with standard oleanolic acid. The proposed method also showed high degree of reproducibility. From the results of scanning electron microscopy a new synergistic phenomenon of heat transfer and mass transfer was proposed.

Keywords
Gymnema sylvestre; Oleanolic acid; Microwave-assisted extraction; Synergistic; Antioxidant; Extraction time
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Design and performance evaluation of a microwave based low carbon yielding extraction technique for naturally occurring bioactive triterpenoid: Oleanolic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issues 1–2, 15 June 2010, Pages 63–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us