fulltext.study @t Gmail

Multi-objective process optimization and integration for the sequential and increased production of biomass, lipase and endospores of a probiotic bacterium

Paper ID Volume ID Publish Year Pages File Format Full-Text
3969 201 2010 5 PDF Available
Title
Multi-objective process optimization and integration for the sequential and increased production of biomass, lipase and endospores of a probiotic bacterium
Abstract

The objective of this study was to substantially enhance the yields of lipase, biomass and spores from a sporogenous probiotic bacterium, Bacillus coagulans RK-02 by multivariate response surface modeling and genetic algorithm based optimization. The effect of temperature, agitation and aeration on time course of growth, lipase formation and sporulation of individual batch cultivation were also studied. The optimum conditions for three responses were found to be different from each other. Comparatively lower temperature and higher agitation and aeration were needed for biomass and lipase production than that for maximizing sporulation rate. In the final validation experiment, three different optimal conditions for maximizing each of these responses, namely biomass, lipase and spore yields, in a stage wise manner were maintained respectively. This strategy produced 6.25 g L−1 biomass, 6 × 1012 spores per gram of biomass, and maximum of 13.46 IU lipase. Such high yield of biomass, lipase and spore from batch cultivation is first ever to be reported.

Keywords
Bacillus coagulans RK-02; Probiotic; Biomass; Spore; Lipase; Multivariate optimization
First Page Preview
Multi-objective process optimization and integration for the sequential and increased production of biomass, lipase and endospores of a probiotic bacterium
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issues 1–2, 15 June 2010, Pages 77–81
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us