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Screening of agro-industrial wastes to produce ligninolytic enzymes by Phanerochaete chrysosporium

Paper ID Volume ID Publish Year Pages File Format Full-Text
3983 202 2010 7 PDF Available
Title
Screening of agro-industrial wastes to produce ligninolytic enzymes by Phanerochaete chrysosporium
Abstract

Ligninolytic enzyme production by solid-state cultures of Phanerochaete chrysosporium BKM-F-1767 was investigated by employing different agro-industrial wastes, such as fishery residues, brewery waste, apple waste (pomace) and pulp and paper industry sludge. Different enzyme inducers, such as veratryl alcohol, Tween-80 and CuSO4 at concentrations of 2 mM, 0.5% (v/w) and 3 mmole/kg, respectively were also tested. Use of veratryl alcohol and Tween-80 resulted in maximum manganese peroxidase (MnP) activity of 17.36 ± 0.5, 540.2 ± 5.1, 631.25 ± 14, and 507.5 ± 26.87 U/gds (units/gram dry substrate), respectively, for different wastes. Maximum lignin peroxidase (LiP) activity of 141.38 ± 3.39 and 14.1 ± 0.5 U/gds was attained with pomace and pulp and paper, respectively. Laccase activities were found to be insignificant for all wastes. Addition of Tween-80 and CuSO4 resulted in highest values of MnP activity of 17.4 ± 0.6, 291 ± 2.8, 213.5 ± 3, and 213.2 ± 3.2 U/gds for fishery waste, brewery waste, pomace and pulp and paper industry sludge, respectively. Addition of CuSO4 to the culture medium enhanced laccase activity. Maximum laccase activities of 738.97 ± 9.2, 719.97 ± 14.6, 308.8 ± 12.1, and 94.44 ± 1.2 U/gds were obtained for brewery waste, pomace, pulp and paper industry sludge and fishery waste, respectively. Brewery wastes and pomace served as excellent sources for the production of MnP, LiP and laccases.

Keywords
ATCC, American type culture collection; cfu, colony forming unit; gds, gram dry substrate; LiP, lignin peroxidase; MnP, manganese peroxidase; MPN, most probable number; OD, optical density; PAP, pulp and paper industry sludge; PDA, potato–dextrose–agar; S
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 3, 15 May 2010, Pages 388–394
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Any Questions? feel free to contact us