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Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
3984 202 2010 6 PDF Available
Title
Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation
Abstract

Luciferase of the soft coral Renilla reniformis (Rluc) has found a multitude of potential applications. However, its production in a secreted form has been solely performed in mammalian cells. In this work, a high production of secreted Rluc was approached in Bacillus subtilis by implementing the fermentation strategy. First, three critical medium components among others were selected by the Plackett–Burman design. Followed by the Box–Behnken design, the medium formulation was optimized and composed of 1.17% glucose, 2.27% yeast extract, and 0.55% glutamate. With the optimum medium, the shake-flask culture of the producer strain was carried out to secret Rluc with the yield of 55 mg/L. Finally, the production scheme was scaled up using a 5-L jar fermenter operated in a fed-batch mode. By controlling the operational condition, the feeding solution was pumped in an impulse mode to the culture. As a consequence, the production of Rluc reaching 500 mg/L was obtained after fermentation for 27 h.

Keywords
Renilla luciferase; Experimental design; Fed-batch fermentation; Bacillus subtilis
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Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 3, 15 May 2010, Pages 395–400
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us