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Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica

Paper ID Volume ID Publish Year Pages File Format Full-Text
3986 202 2010 6 PDF Available
Title
Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica
Abstract

d-Amino acid oxidase from Rhodosporidium toruloides (RtDAO) and Fe3O4 magnetic nanoparticles were encapsulated simultaneously within biomimetic silica, as mediated by polyallylamine. The capacity for this enzyme reached 193 mg/g of biomimetic silica when 15 mg/ml RtDAO was used during encapsulation; the average encapsulation efficiency was approximately 74%. The Tm value (the temperature at which 50% of the initial activity was retained after 1 h of incubation) was increased from 44.3 °C of the free RtDAO to 57.7 °C, clearly indicating the thermal stability was improved by encapsulation. In the presence of 50 mM hydrogen peroxide, encapsulated RtDAO had a half-life of 148 min, an approximately 2-fold increase in resistance to hydrogen peroxide as compared to 78-min half-life of the free form. The encapsulation process is simple and can be completed within minutes; besides, the resultant enzymes can be recovered easily under magnetic field. Such preparation of encapsulated d-amino acid oxidase could be exploited for many potential applications.

Keywords
d-Amino acid oxidase; Biomimetic silica; Enzyme technology; Immobilized enzymes; Protein recovery; Kinetic parameters
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Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 3, 15 May 2010, Pages 408–413
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us