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Buffer interactions: Densities and solubilities of some selected biological buffers in water and in aqueous 1,4-dioxane solutions

Paper ID Volume ID Publish Year Pages File Format Full-Text
4006 203 2009 11 PDF Available
Title
Buffer interactions: Densities and solubilities of some selected biological buffers in water and in aqueous 1,4-dioxane solutions
Abstract

The densities of aqueous 1,4-dioxane solutions of tris(hydroxymethyl)aminomethane (TRIS), N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (TAPS), N-[tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid (TAPSO), and N-tris[hydroxymethyl]-4-amino-butanesulfonic acid (TABS) were measured at T = 298.15, 303.15, 310.15, and 318.15 K, using a high precision vibrating tube digital densitometer. On the basis of these data, the isobaric thermal expansibilities, α(m, T), have been evaluated. The solubilities of these buffers in water and at several concentrations of 1,4-dioxane at 298.15 K have also been determined from the experimental results of density measurements. Two-liquid phase behavior was found from the binary system containing the TABS buffer over a given composition range. The solubility data were further used to calculate the free energy of transfer (ΔG′tr) of these buffers from water to aqueous 1,4-dioxane solutions at 298.15 K. We observed positive values of (ΔG′tr) for the buffers from water to 1,4-dioxane, indicating that the interactions between these biological buffers and 1,4-dioxane are unfavorable. The contribution of transfer free energies (Δg′tr) of various functional groups from water to the aqueous 1,4-dioxane solutions, have been estimated.

Keywords
Enzyme; Protein engineering; Cell engineering; Biomaterials engineering; Biological buffers interactions; Transfer free energy
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Buffer interactions: Densities and solubilities of some selected biological buffers in water and in aqueous 1,4-dioxane solutions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 3, 1 November 2009, Pages 334–344
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us