fulltext.study @t Gmail

A new high-throughput screening method for determining active and enantioselective hydrolases

Paper ID Volume ID Publish Year Pages File Format Full-Text
4007 203 2009 5 PDF Available
Title
A new high-throughput screening method for determining active and enantioselective hydrolases
Abstract

A new high-throughput screening method using fluorescein sodium salt as an indicator to obtain hydrolases with high enantioselectivity is developed, which is demonstrated to be sensitive and reliable. The results determined by the method correlate well with those from GC analysis. This method can be applied to determine activity and enantioselectivity of not only lipase and esterase, but also other enzymes which catalyze hydrolysis reaction releasing proton, such as the protease or amidase. Because of the application of small amount of optically pure enantiomers, screening large libraries of enzymes is allowed at low cost and in short time.

Keywords
Biocatalyst preparation; Enzyme; Microbial; Protein engineering; High-throughput screening; Fluorescein sodium salt
First Page Preview
A new high-throughput screening method for determining active and enantioselective hydrolases
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 3, 1 November 2009, Pages 345–349
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us