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Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
4010 204 2008 5 PDF Available
Title
Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate
Abstract

An enzyme-coupled system, containing a ketoreductase for prochiral ketones reduction and a mannitol dehydrogenase for NADPH regeneration, was discovered from the cell-free extract of Rhodotorula sp. AS2.2241 and used successfully for the asymmetric reduction of 2-acetylpyridine, giving (S)-1-(2-pyridine)ethanol in >99% ee with recycling of NADPH for 4220 times. Moreover, the endogenously coupled enzymes system could also reduce various aromatic ketones such as 3- and 4-acetylpyridines with 96–99% ee.

Keywords
Biocatalysis; Biotransformations; Chromatography; Optimization; NADPH regeneration; Rhodotorula sp. AS2.2241 ketoreductase
First Page Preview
Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 42, Issue 1, 15 October 2008, Pages 1–5
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering