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Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
4016 204 2008 6 PDF Available
Title
Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor
Abstract

For the complete hydrolysis of cellulose, the cellulolytic fungi produce a whole set of commercially important enzymes called cellulases. The aim of this work was to investigate an approach to enhance the production of these enzymes by co-culturing Trichoderma reesei and Aspergillus niger in a bioreactor to convert cellulose substrate into soluble sugars through a synergetic action of enzyme complex simultaneously produced by these two fungi. The experiments were conducted as fed batch growth on a Cellulose–Yeast extract medium. A mixture of lactose and lactobionic acid was added into the bioreactor as cellulase inducers. The results of mixed culture experiments exhibited a highly significant increase in the production of volumetric enzyme activity (98.4 U L−1 h−1), filter paper activity (7.1 U mL−1), carboxymethyl cellulase activity (4.7 U mL−1), soluble proteins (2.1 mg mL−1), dry biomass (21.4 g−1 L−1), and percentage of utilized cellulose (89.4%) as compared with A. niger monocultures.

Keywords
Cellulose; Lactose; T. reesei; A. niger; Cellulase enzymes; Mixed culture
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Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 42, Issue 1, 15 October 2008, Pages 41–46
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us