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Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
4021 204 2008 7 PDF Available
Title
Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis
Abstract

A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8–1.5 g l−1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity.

Keywords
Sequential injection analysis; Sorbitol; Batch cultivation; Pichia pastoris; Mixed substrates
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Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 42, Issue 1, 15 October 2008, Pages 77–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Any Questions? feel free to contact us