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Metabolic regulation of Escherichia coli and its glnG and zwf mutants under nitrogen limitation

Paper ID Volume ID Publish Year Pages File Format Full-Text
4039 205 2010 7 PDF Available
Title
Metabolic regulation of Escherichia coli and its glnG and zwf mutants under nitrogen limitation
Abstract

The effect of nitrogen (N)-limitation on the metabolism of the wild type Escherichia coli BW25113, its glnG, and zwf mutants was investigated based on the chemostat cultures at the dilution rate of 0.2 h−1 under both carbon (C)-limitation and N-limitation. It was shown that gdhA gene expression was down-regulated, and glutamine synthetase-glutamate synthase (GS/GOGAT) pathway was mainly utilized under N-limitation in E. coli BW25113. It was also shown that the specific glucose consumption rate and the specific CO2 evolution rate (CER) were higher in glnG mutant as compared to the wild type under C-limitation. It was also shown that GOGAT is the main pathway for N assimilation under C-limitation, while GDH is the main pathway under N-limitation in glnG mutant. In zwf mutant, glnA, glnG, and gltD gene expressions were significantly down-regulated, while malic enzyme was significantly activated for NADPH production under both C-limitation and N-limitation.

Keywords
Escherichia coli; glnG knockout; Glutamate synthesis pathway; Nitrogen assimilation; zwf knockout
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Metabolic regulation of Escherichia coli and its glnG and zwf mutants under nitrogen limitation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 2, 15 January 2010, Pages 230–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us