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Potential use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone–butanol–ethanol production from cassava starch

Paper ID Volume ID Publish Year Pages File Format Full-Text
4043 205 2010 8 PDF Available
Title
Potential use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone–butanol–ethanol production from cassava starch
Abstract

A high amylase producing Bacillus subtilis WD 161 was used in a co-culture with Clostridium butylicum TISTR 1032 to enhance acetone–butanol–ethanol (ABE) production from starch. The mixed culture of C. butylicum TISTR 1032 and B. subtilis WD 161 without anaerobic pretreatment by reducing agent and N2 flushing increased amylase activity 10 folds and enhanced ABE production 5.4 and 6.5 folds from soluble starch and cassava starch, respectively, compared to those of the pure culture of Clostridium itself. The medium optimization for ABE production by the mixed culture without anaerobic pretreatment found that cassava starch concentration of 40 g/L, C/N ratio of 4.35 and the mixed nitrogen sources of 265 mM yeast extract and 100 mM NH4NO3 gave the highest ABE production in terms of final concentration and productivity. The benefits of using this high amylase producing aerobic Bacillus in a co-culture with anaerobic Clostridium were not only increasing substrate utilization and ABE production but there was also no requirement to add any costly reducing agent to the medium or flushing with N2 to ensure anaerobic condition. This thus makes the anaerobic fermentation more economical and cost effective. This co-culture system may contribute greatly to developing industrialized ABE production.

Keywords
Acetone–butanol–ethanol fermentation; Bacillus subtilis; Cassava starch; Clostridium butylicum; Mixed culture
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Potential use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone–butanol–ethanol production from cassava starch
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 2, 15 January 2010, Pages 260–267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us