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Optimization of inulin hydrolysis by inulinase accounting for enzyme time- and temperature-dependent deactivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
4057 206 2009 6 PDF Available
Title
Optimization of inulin hydrolysis by inulinase accounting for enzyme time- and temperature-dependent deactivation
Abstract

In the present work a deactivation model for an inulinase from Aspergillus niger is presented; a first order kinetic is found and the deactivation constant kd is related to temperature through the Arrhenius model. The deactivation model was satisfactorily validated and implemented into a kinetic model for inulin hydrolysis predictions; the result is a complete model that is able to predict reaction performances for substrate concentrations ranging between 10 and 40 g/l and reaction temperature up to 60 °C, even on a long time scale. The model is also shown to be a powerful tool to understand reaction paths and to choose the optimal reaction conditions for long time scale processes. A relevant problem for enzymatic process temperature optimization is formulated and solved by means of the predictive model determined.

Keywords
Kinetic model; Enzyme deactivation; Fructose production; Inulin hydrolysis; Process optimization; Thermal effects
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Optimization of inulin hydrolysis by inulinase accounting for enzyme time- and temperature-dependent deactivation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 1, 15 December 2009, Pages 81–86
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us