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Functional characterization of a recombinant thermostable xylanase from Pichia pastoris: A hybrid enzyme being suitable for xylooligosaccharides production

Paper ID Volume ID Publish Year Pages File Format Full-Text
4058 206 2009 6 PDF Available
Title
Functional characterization of a recombinant thermostable xylanase from Pichia pastoris: A hybrid enzyme being suitable for xylooligosaccharides production
Abstract

The hybrid xylanase gene Xyn2-A2 was previously constructed and expressed in Pichia pastoris. In this study, the hybrid recombinant enzyme (PTXC2) was prepared from a 2-L bioreactor and subsequently characterized. The produced xylanase was estimated by SDS-PAGE to be 26 kDa and practically free of cellulolytic activity. The optimal temperature and pH of PTXC2 was 65 °C and pH 6.0, respectively. The hybrid enzyme was stable at 60 °C and retained more than 85% of its activity after 30 min incubation at this temperature. The specificity of PTXC2 towards different natural substrates was evaluated. PTXC2 was highly specific towards xylans tested but exhibited low activities towards cellulosic substrates, such as gellan gum (9.7%), Avicel (1.5%) and carboxymethylcellulose (CMC, 1.2%). The apparent Km values of oat-spelt xylan and birchwood xylan was 1.6 and 1.8 mg/mL, respectively. Analysis of xylan hydrolysis products confirmed as expected that the enzyme functions as an endo-xylanase with xylotriose as the main hydrolysis products. These attributes should make the enzyme an attractive applicant for various applications, such as large-scale production of xylooligosaccharides.

Keywords
Xylanase; Expression; Characterization; Thermostability; Xylooligosaccharides
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Functional characterization of a recombinant thermostable xylanase from Pichia pastoris: A hybrid enzyme being suitable for xylooligosaccharides production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 1, 15 December 2009, Pages 87–92
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us