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Influence of pH and acid solutes on the phase behaviour of aqueous solutions containing poly(ethylene glycol) and poly(ethyleneimine)

Paper ID Volume ID Publish Year Pages File Format Full-Text
4061 206 2009 7 PDF Available
Title
Influence of pH and acid solutes on the phase behaviour of aqueous solutions containing poly(ethylene glycol) and poly(ethyleneimine)
Abstract

In this paper, which forms part of a study on aqueous two-phase systems (ATPS) for separation of low-molar-mass organic acids in the biotechnology industry, we examine the phase behaviour of ATPSs containing poly(ethylene glycol) (PEG) and poly(ethyleneimine) (PEI) as a function of pH, the nature of the acid used for titrating PEI, and the addition of lactic acid. We show that increasing the pH leads to contraction of the two-phase regions and that titrating PEI with a higher polyvalent acid results in a larger two-phase region. We propose a mechanism explaining the experimentally observed phase behaviour. Finally, we demonstrate that the lactic acid partition coefficient is very favourable (the acid partitions preferentially to the PEI-rich phase), which confirms that the (PEG + PEI) ATPSs could be used to advantage as a medium for lactic acid bio-transformation.

Keywords
ATPS; Lactic acid; Partition coefficient
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Influence of pH and acid solutes on the phase behaviour of aqueous solutions containing poly(ethylene glycol) and poly(ethyleneimine)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 1, 15 December 2009, Pages 104–110
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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