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A new salt inducible expression system for Lactococcus lactis

Paper ID Volume ID Publish Year Pages File Format Full-Text
4065 206 2009 4 PDF Available
Title
A new salt inducible expression system for Lactococcus lactis
Abstract

A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0 μkat l−1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation. The major advantage of the current expression system is that no additions of inducing agents are needed into bioreactor cultivations.

Keywords
Bioreactor systems; BusR; Lactococcus lactis; Salt induction; Recombinant proteins; Expression systems
First Page Preview
A new salt inducible expression system for Lactococcus lactis
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 1, 15 December 2009, Pages 132–135
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering