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Immobilization of β galactosidase from Aspergillus oryzae via immunoaffinity support

Paper ID Volume ID Publish Year Pages File Format Full-Text
4081 207 2009 8 PDF Available
Title
Immobilization of β galactosidase from Aspergillus oryzae via immunoaffinity support
Abstract

Polyclonal antibody bound cellulose support has been exploited for the immobilization and stabilization of β galactosidase from Aspergillus oryzae. Immunoaffinity bound β galactosidase retained 96.5% of the initial activity on the support. Immobilized β galactosidase showed broad-spectrum pH optima, pH 4.6–5.5 and temperature at 50–60 °C whereas the soluble enzyme exhibited activity peak at pH 4.6 and 50 °C. Immunoaffinity bound enzyme preparation was quite stable to thermal denaturation and it retained 72% activity after 4 h incubation at 60 °C whereas under these conditions the soluble β galactosidase lost almost its full activity. IgG-cellulose immobilized β galactosidase was quite stable against the inactivation caused by proteolytic enzymes; trypsin and pepsin. Moreover, this immobilized enzyme preparation retained 64%, 71% and 65% activity in the presence of 4.0 M urea, 5% CaCl2 and 5% galactose. IgG-cellulose bound β galactosidase exhibited 80% of its original activity after 2 months storage at 4 °C while the soluble enzyme showed only 35% of the initial activity. After 10th repeated use immobilized β galactosidase retained 46% activity.

Keywords
DEAE, diethyl aminoethyl; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulphate; ELISA, enzyme linked immunosorbent assay; LB, Langmuir–Blodgett; ONPG, o-nitrophenyl β-d-galactopyranoside; TBS, Tris buffer salineβ Galactosidase; Cellulose
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Immobilization of β galactosidase from Aspergillus oryzae via immunoaffinity support
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 3, 15 March 2009, Pages 307–314
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us