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Self-interaction of native and denatured lysozyme in the presence of osmolytes, l-arginine and guanidine hydrochloride

Paper ID Volume ID Publish Year Pages File Format Full-Text
4083 207 2009 6 PDF Available
Title
Self-interaction of native and denatured lysozyme in the presence of osmolytes, l-arginine and guanidine hydrochloride
Abstract

Osmolyte molecules such as betaine and trehalose are protein stabilizers while l-arginine (Arg) and guanidine hydrochloride (GdnHCl) are the most widely used aggregation suppressor in protein refolding. We have herein studied the effects of the osmolyte molecules and l-arginine together with GdnHCl (0–6 mol/L) on the intermolecular interaction of native and denatured lysozyme by self-interaction chromatography. The self-interaction is characterized in terms of the osmotic second virial coefficient (B) of the protein, the increase of which represents the decrease of intermolecular attraction of the protein. It is found that the effect of Arg on the self-interaction of lysozyme is similar with GdnHCl, but its competence is much weaker than the denaturant. At higher GdnHCl concentrations (>0.5 mol/L), Arg can be used to suppress the self-association of lysozyme. In contrast to Arg, B increases with increasing betaine or trehalose concentration at the GdnHCl concentration range studied. The results indicate the cooperativity of each osmolyte with GdnHCl, and the different mechanisms of their effects from Arg on the B values. The work confirms that the osmolytes are not only protein stabilizers, but also protein aggregation suppressors for both native and denatured protein molecules.

Keywords
Bioseparations; Protein refolding; Chromatography; Affinity; Self-interaction; Second virial coefficient
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Self-interaction of native and denatured lysozyme in the presence of osmolytes, l-arginine and guanidine hydrochloride
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 3, 15 March 2009, Pages 321–326
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us