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Synthesis of ethyl butyrate in organic media catalyzed by Candida rugosa lipase immobilized in polyurethane foams: A kinetic study

Paper ID Volume ID Publish Year Pages File Format Full-Text
4084 207 2009 6 PDF Available
Title
Synthesis of ethyl butyrate in organic media catalyzed by Candida rugosa lipase immobilized in polyurethane foams: A kinetic study
Abstract

A kinetic study on the synthesis of ethyl butyrate in n-hexane, catalyzed by Candida rugosa lipase immobilized in two hydrophilic polyurethane foams (“HYPOL FHP 2002” and “HYPOL FHP 5000”) was performed. With the “FHP5000” foams, esterification rates and conversion were always higher than those obtained with “FHP2002”. For both immobilized preparations, BA did not cause any inhibition on the enzymatic activity, in the range of concentration tested (0.078–0.7 M) at an initial ethanol concentration of 0.105 M. Michäelis–Menten kinetics was observed: a plateau being reached at the initial bulk BA concentration of 0.40 M and 0.45 M, corresponding to microenvironmental concentrations of 0.851 M and 0.329 M, respectively with the lipase in “FHP2002” and “FHP5000” foams. Inhibition by EtOH was observed for initial bulk concentrations higher than 0.15 M, corresponding to microenvironmental concentrations of 0.426 M and 0.256 M, for the lipase in “FHP2002” and “FHP5000” foams, respectively. Kinetic data could be well described by the substrate-inhibition model, considering the initial bulk or microenvironmental ethanol concentrations as inhibitory.

Keywords
Biocatalyst preparation; Enzyme; Immobilized; Kinetics; Lipase; Polyurethane foams
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Synthesis of ethyl butyrate in organic media catalyzed by Candida rugosa lipase immobilized in polyurethane foams: A kinetic study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 3, 15 March 2009, Pages 327–332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us