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Functional polymeric supports for immobilization of cholesterol oxidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
4085 207 2009 5 PDF Available
Title
Functional polymeric supports for immobilization of cholesterol oxidase
Abstract

Here, we have reported the useful functional polymeric supports for possible application of enzyme immobilization. Functional polymers were prepared by free radical polymerization from different monomers (i.e., methylmetacrylate, glycidylmethacrylate, acrylamide, etc.) and N,N-methylenebis(acrylamide) (MBAAm) crosslinker. Cholesterol oxidase (ChOx) [EC.1.1.3.6] was then covalently immobilized onto these functional supports via epichlorohydrin (ECH) and carbodiimide (EDAC) as the activating agents. It was observed that, after 60th use in 5 days, the retained activities for immobilized enzymes onto poly(methyl methacrylate-co-glycidyl methacrylate) [P(MMA-co-GMA)] and poly(acrylamide-co-acrylic acid)/polyethyleneimine [P(AAm-co-AA)/PEI] supports were found as 56% and 83%, respectively.

Keywords
Immobilization; Kinetic parameters; Enzyme stability; Biosensors; Biomaterials; Cholesterol oxidase
First Page Preview
Functional polymeric supports for immobilization of cholesterol oxidase
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 3, 15 March 2009, Pages 333–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering