fulltext.study @t Gmail

The effect of pendant hydrophobicity on the biological efficacy of polyethylenimine conjugate

Paper ID Volume ID Publish Year Pages File Format Full-Text
4091 208 2010 7 PDF Available
Title
The effect of pendant hydrophobicity on the biological efficacy of polyethylenimine conjugate
Abstract

Modifying cationic polymers by conjugating a pendant group is a commonly used approach for improving the biological efficacy of gene delivery. Polyethylenimine (PEI) of MW 25,000, a potent cationic polymer for gene delivery, was employed as the primary carrier. Three different types of PEI conjugates were prepared by grafting cellobiose, polyethylene glycol of MW 350 (PEG), and 1-iodododecane, respectively, to assess the hydrophobic effect on the biological efficacy and chemicophysical properties of the DNA–polymer complexes. Increasing the hydrophobicity of the conjugate was found to decrease the biological efficacy including the levels of transgene expression and cellular viability. When the conjugate hydrophobicity was high enough to form micelles below 0.11 mg/mL, the reduction in transgene expression became moderated but was accompanied with a sharp decrease in cellular viability. On the other hand, when the conjugate hydrophobicity was decreased by conjugating PEG or cellobiose at the optimal % of around 1.2%, the level of transgene expression was enhanced while at the same time maintaining the cellular viability and the chemicophysical properties of the complexes. These results suggested that a hydrophilic pendant group could better promote the biological efficacy of PEI by decreasing the hydrophobicity.

Keywords
Biomaterials engineering; Controlled release; Cell engineering; Biopolymers; Transfection; Non-viral vectors
First Page Preview
The effect of pendant hydrophobicity on the biological efficacy of polyethylenimine conjugate
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 1, 15 March 2010, Pages 21–27
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us