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Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata using statistical designs

Paper ID Volume ID Publish Year Pages File Format Full-Text
4095 208 2010 9 PDF Available
Title
Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata using statistical designs
Abstract

The recombinant strain of the yeast Candida famata 13-76 that overexpresses FMN1 gene coding for riboflavin kinase, accumulates significant amounts of flavin mononucleotide (FMN) in the cultural liquid. The effectiveness of FMN production by this strain under different conditions was evaluated. First, the medium composition was optimized in shake flask cultures. After preliminary experiments for nitrogen source selection, the two-level Plackett–Burman (PB) design was performed to screen medium components that significantly influence the FMN production. Among the 15 variables tested, KH2PO4, CaCl2, (NH4)6Mo7O24, CuSO4 and yeast extract, were identified as the most significant factors for FMN production (confidence levels above 95%). In order to investigate the quantitative effects for five variables selected from PB design on FMN production, a central composite design (CCD) was subsequently employed for further optimization. The optimization strategies used led to a 4.77-fold increase in the FMN production. A batch culture profile in a 1 L fermenter was consequently designed according to the optimal medium observed in shake flasks. A final FMN concentration of 231 ± 4.11 mg/L was obtained in 40 h, which further verified the practicability of the used strategy.

Keywords
Candida famata; Flavin mononucleotide production; Medium optimization; Plackett–Burman design; Central composite design; Response surface methodology
First Page Preview
Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata using statistical designs
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 1, 15 March 2010, Pages 52–60
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering