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Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies

Paper ID Volume ID Publish Year Pages File Format Full-Text
4102 208 2010 9 PDF Available
Title
Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies
Abstract

Lentinus edodes (shiitake mushroom) is a well-known medicinal and edible mushroom throughout the world. Statistical optimization was employed to optimize the culture medium for maximum mycelial growth and exo-polysaccharide (EPS) production in submerged fermentation: Plackett–Burman design (PBD) was applied to determine significant factors, followed by the paths of steepest ascent to move to the general vicinity of the optimum and Box–Behnken design (BBD) to obtain the final optimum culture medium composition. Glucose, yeast-powder and pH were significant for fermentation. 15.4 g glucose and 5.32 g yeast-powder per liter and pH 4.61 were optimum for the biomass accumulation from 2.75 g/l to 6.88 g/l, while 15.78 g glucose and 5.86 g yeast-powder per liter and pH 4.48 were optimum for EPS production from 0.214 g/l to 0.751 g/l. Under the optimized cultivation condition, cultivation kinetic models were studied in flask (pellet growth form) and airlift (filamentous growth form) reactors. The two morphologies resulted in different culture processes and mathematical models.

Keywords
Lentinus edodes; Statistical optimization; Biomass; Exo-polysaccharide; Morphology; Growth kinetics
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Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 49, Issue 1, 15 March 2010, Pages 104–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us