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Fermentative lactic acid production with a metabolically engineered yeast immobilized in photo-crosslinkable resins

Paper ID Volume ID Publish Year Pages File Format Full-Text
4120 209 2009 5 PDF Available
Title
Fermentative lactic acid production with a metabolically engineered yeast immobilized in photo-crosslinkable resins
Abstract

In this study, the immobilization technique involving photo-crosslinkable resin gels was used for lactic acid production. Saccharomyces cerevisiae OC-2T T165R, a metabolically engineered yeast that produces optically pure l(+)-lactic acid, was immobilized in hydrophilic photo-crosslinked resin gels as a biocatalyst. Three resin gels, TEP 1, TEP 2 and TEP 3, were examined and all of them showed high performance as to lactic acid production. Resin gel TEP 1, which exhibited the highest productivity among the resin gels was used for 15 consecutive batch fermentations without decreases in productivity and mechanical deformation, indicating that it was a suitable carrier for long-term lactic acid fermentation. Moreover, the use of the immobilization technique can improve the productivity of the metabolically engineered yeast in the fermentation with or without extraction, showing promise for using the immobilized engineered yeast for lactic acid production.

Keywords
Immobilization; Lactic acid fermentation; Photo-crosslinkable resin gel; Yeast
First Page Preview
Fermentative lactic acid production with a metabolically engineered yeast immobilized in photo-crosslinkable resins
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 47, Issues 1–3, 1 December 2009, Pages 66–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering