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Modification of cellulase and its application to extraction of diosgenin from Dioscorea zingiberensis C.H. Wright

Paper ID Volume ID Publish Year Pages File Format Full-Text
4122 209 2009 7 PDF Available
Title
Modification of cellulase and its application to extraction of diosgenin from Dioscorea zingiberensis C.H. Wright
Abstract

In order to enhance the thermostability and efficiency of cellulase in the extraction of diosgenin from Dioscorea zingiberensis C.H. Wright, we applied polyethylene glycol (PEG) (400, 1000, 2000, and 4000) to modify cellulase. The modified cellulase, α-amylase and β-glycosidase were used to hydrolyze the material. The results show that the thermostability of modified cellulase is better than that of natural cellulase, the optimum pH value and temperature of modified cellulase are wider than that of natural cellulase, the activity of cellulase modified by activated PEG2000 is higher than that of cellulase modified by other modifiers, and its remaining activity is 58% of its initial value. With this technique, the purity of the product reaches 96%, the melting point is 201–204 °C, the yield rate and the extraction rate of the diosgenin reaches 2.80% and 96.6%, respectively. IR spectra and 1H NMR spectroscopy were used to confirm the structure of the product.

Keywords
CC, cyanuric chloride; CC-PEG, the activated cyanuric chloride PEG; CMC, carboxymethylcellulose; FT-IR, Fourier transform infrared spectroscopy; HPLC, high-pressure liquid chromatography; MPEG, mono-methoxy polyethylene glycol; MWCO, molecular weight cut
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Modification of cellulase and its application to extraction of diosgenin from Dioscorea zingiberensis C.H. Wright
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 47, Issues 1–3, 1 December 2009, Pages 80–86
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us