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Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
4135 210 2009 17 PDF Available
Title
Bioreactor engineering for recombinant protein production in plant cell suspension cultures
Abstract

A review of over 15 years of research, development and commercialization of plant cell suspension culture as a bioproduction platform is presented. Plant cell suspension culture production of recombinant products offers a number of advantages over traditional microbial and/or mammalian host systems such as their intrinsic safety, cost-effective bioprocessing, and the capacity for protein post-translational modifications. Recently significant progress has been made in understanding the bottlenecks in recombinant protein expression using plant cells, including advances in plant genetic engineering for efficient transgene expression and minimizing proteolytic degradation or loss of functionality of the product in cell culture medium. In this review article, the aspects of bioreactor design engineering to enable plant cell growth and production of valuable recombinant proteins is discussed, including unique characteristics and requirements of suspended plant cells, properties of recombinant proteins in a heterologous plant expression environment, bioreactor types, design criteria, and optimization strategies that have been successfully used, and examples of industrial applications.

Keywords
Recombinant proteins; Transgenic; Plant cell cultures; Bioreactors; Bioprocess; Optimization
First Page Preview
Bioreactor engineering for recombinant protein production in plant cell suspension cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 45, Issue 3, 15 August 2009, Pages 168–184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering