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Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
4137 210 2009 9 PDF Available
Title
Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production
Abstract

Current ethanol production processes using crops such as sugar cane and corn are well established; however, utilization of a cheaper substrate such as lignocellulose could make bioethanol more competitive with fossil fuel, without the ethical concerns associated with the use of potential food resources. The sequential configuration employed to obtain cellulosic ethanol implies that the solid fraction of pretreated lignocellulosic material undergoes hydrolysis. In this work, the enzymatic hydrolysis of pretreated Cistus ladanifer and Cytisus striatus was studied following an experimental design as a statistical problem solving approach. Plackett–Burman design was used in order to select the most important variables from the simultaneous study on influence of operating and reactional conditions, and central composite design to optimize the process of enzymatic hydrolysis.The optimization of enzymatic hydrolysis using the response surface methodology allowed a study on the influence of the variables (pH, temperature, cellulases concentration, polymer (PEG) concentration and incubation time) and variability due to the type of substrate (C. ladanifer and C. striatus) used. From the obtained results it can be concluded that the enzymatic hydrolysis was clearly enhanced by temperature, cellulase concentration, and incubation time. Model validation showed a good agreement between experimental results and the predicted responses.

Keywords
Enzymatic hydrolysis; Enzyme; Biomass; Lignocellulose degradation; Optimization; Response surface methodology
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Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 45, Issue 3, 15 August 2009, Pages 192–200
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us