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Organic solvent-tolerant enzymes

Paper ID Volume ID Publish Year Pages File Format Full-Text
4147 211 2010 13 PDF Available
Title
Organic solvent-tolerant enzymes
Abstract

The use of organic solvents as reaction media for enzymatic reactions provides numerous industrially attractive advantages compared to traditional aqueous reaction systems. Despite the advantages, native enzymes almost universally exhibit low activities and/or stabilities in the presence of organic solvents. This inactivation of the enzymes by organic solvents results in significant limitation of the enzymatic reaction process. Numerous attempts have been made to improve enzyme activity and stability in the presence of organic solvents using methods based on protein engineering and chemical or physical modification. Most enzymes used in these studies did not originally exhibit high activity and stability in the presence of organic solvents because they were not screened as organic solvent-tolerant enzymes. Recently, various attempts have been made to screen enzymes that naturally possess organic solvent-tolerance form various microorganisms including organic solvent-tolerant bacteria, thermophiles, halophiles and mesophiles. These organic solvent-tolerant enzymes are expected to have potential for applications in industrial chemical processes.

Keywords
Organic solvent-tolerant; Organic solvent-stable; Enzymes; Non-aqueous; Extremophiles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 3, 15 February 2010, Pages 270–282
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Online Support
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