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Practical and functional culture technologies for primary hepatocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
4152 211 2010 5 PDF Available
Title
Practical and functional culture technologies for primary hepatocytes
Abstract

The liver is a central organ for metabolism in the body, and it is therefore important to develop effective culture methods for the expression of liver-specific functions. The development of effective primary hepatocyte culture methods for the expression of these functions has been attempted by focusing on cell–cell and cell–matrix interactions. Two-dimensional organoid culture methods using substrata with the immobilized arginine–glycine–aspartic acid sequence, a well-known cell-adhesive sequence, have been developed. Furthermore, a drug metabolism simulator and a hybrid-type blood purification system as applications of this type of two-dimensional organoid culture method have been developed. On the other hand, growth factor/heparin-immobilized culture substrata and cell-embedded hydrogels in extracellular matrix-filled biocompatible macroporous scaffold culture systems for liver tissue engineering have been developed. Promotion of organoid formation and high expression of the liver-specific functions of primary rat hepatocytes will be realized by using these substrata and culture systems.

Keywords
Animal cell culture; Biomedical; Bioreactor; Hepatocyte; Immobilization; Liver tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 3, 15 February 2010, Pages 332–336
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us