fulltext.study @t Gmail

Application of flow cytometry to industrial microbial bioprocesses

Paper ID Volume ID Publish Year Pages File Format Full-Text
4157 211 2010 23 PDF Available
Title
Application of flow cytometry to industrial microbial bioprocesses
Abstract

Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture, by means of light-scattering and fluorescence measurements. In biotechnology, the power of this method lies both in the possibility of determining a wide range of cell parameters at single cell level, and in the ability to obtain information about their distribution within cell populations, providing valuable information for bioprocess design and control. The first part of this review will be devoted to describe the basic aspects of multicolour FC, the different strategies used for cell detection, and the cellular target sites and fluorescent probes which are currently used in cell assays. Next, a deep revision concerning the most recent FC applications related to cell analysis and quantification (especially both bacteria and yeast) in food, drink and pharmaceutical industries and in natural environment and water systems is presented. This review seeks to highlight the advantages of this technique in microbial fermentations monitoring and control, as well as in the development of more accurate kinetic models directed to bioprocesses optimization. Finally, an overview of some commercial FC devices is presented along with a discussion of the progress and advantages of the method for bioprocesses on-line monitoring.

Keywords
Flow cytometry; Instrumentation; Cell parameters; Bioprocesses control; Optimization; Kinetic modelling
First Page Preview
Application of flow cytometry to industrial microbial bioprocesses
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 48, Issue 3, 15 February 2010, Pages 385–407
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us