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Sequential simplex optimization of recombinant biotinylated survivin production by Escherichia coli using mineral supplementation

Paper ID Volume ID Publish Year Pages File Format Full-Text
4166 212 2009 6 PDF Available
Title
Sequential simplex optimization of recombinant biotinylated survivin production by Escherichia coli using mineral supplementation
Abstract

The sequential simplex method is a useful tool for rapidly optimizing a process by moving through factor space via a relatively simple geometric algorithm. This method was employed to optimize growth and survivin-BCCP production in Escherichia coli Origami B using zinc sulphate supplemented culture medium. Five experimental parameters were investigated: concentration of zinc sulphate and IPTG, pH, temperature, and agitation rate. Optimized conditions were as follows: 190 μM zinc sulphate with 246 μM IPTG, pH 7, at a temperature of 23.5 °C and agitation rate of 345 rpm. The results for cell density and survivin-BCCP production were, 17% and 140% higher than in non-zinc supplemented medium and 12.6% and 25.5% higher than could be obtained by varying one-factor-at-a-time. The present work demonstrates the effectiveness of zinc sulphate supplementation and the efficiency of the sequential simplex optimization method in obtaining high yields.

Keywords
Sequential simplex optimization; E. coli Origami B strain; Recombinant protein production; Biotinylated survivin; Process optimization
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Sequential simplex optimization of recombinant biotinylated survivin production by Escherichia coli using mineral supplementation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 115–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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