fulltext.study @t Gmail

Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil

Paper ID Volume ID Publish Year Pages File Format Full-Text
4167 212 2009 5 PDF Available
Title
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
Abstract

Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10−4 and 6.4 × 10−4 mg mL−1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.

Keywords
Micellar electrokinetic chromatography; Alkaline phosphatase; Acid phosphatase; Enzyme activity; Adenosine monophosphate
First Page Preview
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 121–125
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us