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Iron oxide-chitosan hybrid nanobiocomposite based nucleic acid sensor for pyrethroid detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
4169 212 2009 9 PDF Available
Title
Iron oxide-chitosan hybrid nanobiocomposite based nucleic acid sensor for pyrethroid detection
Abstract

Nucleic acid sensor has been fabricated via immobilization of single standard calf thymus deoxyribose nucleic acid (ssCT-DNA) onto chitosan (CH)-iron oxide (Fe3O4) nanoparticles based hybrid nanobiocomposite film deposited onto indium-tin-oxide (ITO) coated glass for pyrethroids [cypermehtirn (CM) and permethrin (PM)] detection. The ssCT-DNA immobilized onto CH-Fe3O4 nanocomposite/ITO electrode has been characterized using scanning electron microscopy (SEM), UV–visible, Fourier transform infrared (FTIR) spectroscopy, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) techniques. This disposable ssCT-DNA/CH-Fe3O4 nanobiocomposite/ITO bioelectrode is stable for about two months under refrigerated conditions and can detect CM (0.0025–2 ppm) and PM (1–300 ppm) using DPV technique within 25 s and 40 s, respectively.

Keywords
Biosensor; Biocatalysis; Biopolymers; Fe3O4 nanoparticles; Nanobiocomposite; Electrochemical detection
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Iron oxide-chitosan hybrid nanobiocomposite based nucleic acid sensor for pyrethroid detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 132–140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us