fulltext.study @t Gmail

Influence of temperature on the activity and enantioselectivity of Burkholderia cepacia lipase in the kinetic resolution of mandelic acid enantiomers

Paper ID Volume ID Publish Year Pages File Format Full-Text
4171 212 2009 7 PDF Available
Title
Influence of temperature on the activity and enantioselectivity of Burkholderia cepacia lipase in the kinetic resolution of mandelic acid enantiomers
Abstract

The effect of temperature on Burkholderia cepacia lipase (Amano PS) activity and enantioselectivity in the kinetic resolution of mandelic acid has been investigated. Experiments were carried out at temperatures between 25 and 60 °C. The Bi Bi Ping Pong model has been used to calculate kinetic parameters of the transesterification of mandelic acid by vinyl acetate. The rate of transesterification of R enantiomer was found to be substantially lower than that of S enantiomer. A decrease in the reaction rates was observed in temperature above 55 °C. The equilibrium model of enzyme deactivation was used to describe the dependence of mandelic acid transesterification rate on temperature. The enthalpy and entropy of the enzyme inactivation have been estimated. The enantioselectivity, expressed as enantiomeric ratio (ηE), was very high and decreased a little with the temperature increase. The transition state theory was used to determine the thermodynamic activation parameters. The (S)-mandelic acid transesterification has been found to be enthalpically and entropically favoured. The differentiation of mandelic acid enantiomers in the reaction conditions is driven by the difference in activation enthalpy and entropy as well as by the energy of the substrate binding.

Keywords
Enzyme; Kinetics; Microbial lipase; Temperature effect; Thermodynamic parameters; Protein engineering
First Page Preview
Influence of temperature on the activity and enantioselectivity of Burkholderia cepacia lipase in the kinetic resolution of mandelic acid enantiomers
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 147–153
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us