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Immobilized transglucosidase in biomimetic polymer–inorganic hybrid capsules for efficient conversion of maltose to isomaltooligosaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
4176 212 2009 7 PDF Available
Title
Immobilized transglucosidase in biomimetic polymer–inorganic hybrid capsules for efficient conversion of maltose to isomaltooligosaccharides
Abstract

Isomaltooligosaccharides (IMOs) are relatively new functional food ingredients which have great potential to improve the quality of many foods due to their low calories, no cariogenicity and safety for diabetics. To convert maltose to IMOs efficiently, α-transglucosidase was immobilized in a kind of alginate–chitosan–calcium phosphate hybrid capsules (Alg–Chi–CaP), which were prepared through a facile bio-inspired mineralization process. The surface morphology of Alg–Chi–CaP capsule and alginate–chitosan capsule (Alg–Chi) was characterized by scanning electron microscopy (SEM). Due to the presence of inorganic shell, immobilization efficiency of transglucosidase in Alg–Chi–CaP capsules was higher than that in Alg–Chi capsules. The optimal temperature (60 °C) and pH (6.0) value for enzymatic conversion catalyzed by transglucosidase immobilized Alg–Chi–CaP capsules were identical to those catalyzed by free transglucosidase. As compared to the free enzyme, transglucosidase in Alg–Chi–CaP capsules exhibited significantly higher recycling stability and storage stability in a broader temperature and pH range.

Keywords
Enzyme; Immobilized; Biocatalyst preparation; Kinetics; Biomimetic hybrid capsule; Isomaltooligosaccharide
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Immobilized transglucosidase in biomimetic polymer–inorganic hybrid capsules for efficient conversion of maltose to isomaltooligosaccharides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 186–192
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us