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Primary rat hepatocytes form spheroids on hepatocyte growth factor/heparin-immobilized collagen film and maintain high albumin production

Paper ID Volume ID Publish Year Pages File Format Full-Text
4183 212 2009 7 PDF Available
Title
Primary rat hepatocytes form spheroids on hepatocyte growth factor/heparin-immobilized collagen film and maintain high albumin production
Abstract

We developed hepatocyte growth factor (HGF)/heparin-immobilized collagen film by adding HGF solution to collagen film, which immobilized heparin by chemical cross-linking. Primary rat hepatocytes maintained neither their specific morphology nor albumin synthesis on collagen film that directly immobilized HGF by cross-linking. By using HGF-supplemented medium, hepatocytes on collagen film formed typical monolayers and maintained albumin synthesis. Hepatocytes on HGF/heparin-immobilized collagen film formed spheroids after 3 days. They maintained albumin production during the culture period, the same as in HGF-supplemented medium. However, spheroids were not formed although the adhesion of hepatocytes was disturbed on heparin-immobilized collagen film, and albumin synthesis rapidly decreased during 5 days of culture. Because the mitochondrial activities of hepatocytes were not increased in all conditions, it was supposed that the cell proliferation did not occur. Therefore, it was shown that HGF/heparin-immobilized collagen film was effective for promotion of spheroid formation of hepatocytes and expression of their albumin production.

Keywords
Hepatocyte growth factor; Heparin; Tissue engineering; Artificial organs; Animal cell culture engineering; Cell engineering
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Primary rat hepatocytes form spheroids on hepatocyte growth factor/heparin-immobilized collagen film and maintain high albumin production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 227–233
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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