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Lipase-immobilized electrospun PAN nanofibrous membranes for soybean oil hydrolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
4192 213 2009 6 PDF Available
Title
Lipase-immobilized electrospun PAN nanofibrous membranes for soybean oil hydrolysis
Abstract

Polyacrylonitrile (PAN) nanofibrous membranes were prepared by electrospinning and activated by amidination reaction for immobilizing Candida rugosa lipase with covalent binding. The diameters of initial PAN nanofibers ranged from 150 to 300 nm. The lipase, which aggregated and formed small particles that attached onto the nanofiber surfaces, was observed using field emission scanning electron microscopy. The activity retention of the immobilized lipase was 87.5% of the free enzyme. The kinetic parameters, Km and Vmax, of the immobilized lipase were determined to be 0.548 mM and 31.2 U/mg, respectively. The effects of oil-to-water ratio, temperature, and pH on hydrolysis of soybean oil were investigated to determine the optimal reaction conditions. Under these optimal reaction conditions, the hydrolysis conversion of soybean oil was 72% after 10 min and 85% after 1.5 h. In examining reusability, the immobilized lipase retained 65% of its initial conversion after 20 additional batch reactions. These results suggest that the proposed scheme for immobilization of lipase has potential in industrial applications for oil hydrolysis.

Keywords
Nanofibrous membrane; Electrospinning; Lipase; Immobilized; Kinetic parameter; Hydrolysis
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Lipase-immobilized electrospun PAN nanofibrous membranes for soybean oil hydrolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 45, Issue 1, 1 June 2009, Pages 48–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us