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Enhanced conductometric immunoassay for hepatitis B surface antigen using double-codified nanogold particles as labels

Paper ID Volume ID Publish Year Pages File Format Full-Text
4204 214 2009 6 PDF Available
Title
Enhanced conductometric immunoassay for hepatitis B surface antigen using double-codified nanogold particles as labels
Abstract

A new conductometric immunoassay for hepatitis B surface antigen (HBsAg) was developed based bioelectrocatalytic reaction on a microcomb-type electrode by using double-codified nanogold particles as labels. This microcomb-type electrode was fabricated on an interdigitated transducer covered with a well-ordered anti-HBs/protein A/nanogold architecture. The double-codified nanogold particles were prepared by using nanogold-labeled anti-HBs antibodies conjugated with horseradish peroxidase (HRP). Sandwich-type immunoassay protocol was successfully introduced for the detection of HBsAg. The formation of the immunocomplex changed the direct electrical communication between the carried HRP and the electrode, and thus local conductivity variations could be assayed based on the bioelectrocatalytic reaction of the carried HRP in 0.01 M PBS (pH 7.0) containing 60 μM H2O2, 0.08 M KI and 0.1 M NaCl. Under optimized conditions, the linear range obtained by using HRP-conjugated anti-HBs as secondary antibodies was 1.5–450 ng/mL HBsAg, while the assay sensitivity by using double-codified nanogold particles could be further increased to 0.01 ng/mL with the linear range from 0.1 to 600 ng/mL HBsAg. The developed immunoassay method showed good precision, high sensitivity, acceptable stability and reproducibility, and could be used for the detection of real sample with consistent results in comparison with those obtained by the ELISA method.

Keywords
Conductometric immunosensor; Double-codified nanogold particles; Hepatitis B surface antigen; Sandwich-type immunoassay; Microcomb-type electrode; Hepatitis B surface antibody
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 45, Issue 2, 15 July 2009, Pages 107–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us