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Oxidative functionalisation of lignin by layer-by-layer immobilised laccases and laccase microcapsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
42283 45919 2010 9 PDF Available
Title
Oxidative functionalisation of lignin by layer-by-layer immobilised laccases and laccase microcapsules
Abstract

Laccase was either supported onto alumina particles or physically entrapped inside microcapsules. In both cases, the enzyme was protected by coating with oppositely charged polyelectrolytes by means of the layer-by-layer technique. The activities of these laccase particles and microcapsules were studied on softwood milled wood and kraft residual lignins, in the presence and absence of different mediators. Quantitative 31P NMR was used to determine the structural modifications induced in the lignin structures. Compared to the native enzyme, the laccase particles and microcapsules were found to be more reactive, showing higher conversions. Irrespective of the experimental conditions, the oxidation efficiency was enhanced by the presence of 1-hydroxybenzotriazole or violuric acid as the oxidation mediator. Differently immobilised laccases showed distinct reaction selectivities in the oxidative pattern of lignin.

Graphical abstractLaccase was either supported onto alumina particles or physically entrapped inside microcapsules. In both cases, the enzyme was protected by coating with oppositely charged polyelectrolytes by means of the layer-by-layer technique. The activities of these laccase particles and microcapsules were studied on softwood milled wood and kraft residual lignins, in the presence and absence of different mediators. Compared with the native enzyme, the laccase particles and microcapsules were found to be more reactive, showing higher conversions.Figure optionsDownload full-size imageDownload high-quality image (39 K)Download as PowerPoint slide

Keywords
Layer-by-layer self-assembly; Enzyme encapsulation; Polyelectrolyte microcapsules; Laccase; Lignin; 31P NMR
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Oxidative functionalisation of lignin by layer-by-layer immobilised laccases and laccase microcapsules
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Publisher
Database: Elsevier - ScienceDirect
Journal: Applied Catalysis A: General - Volume 372, Issue 2, 15 January 2010, Pages 115–123
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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