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Enantioselective whole-cell biotransformation of acetophenone to S-phenylethanol by Rhodotorula glutinis: Part I. Product formation kinetics and feeding strategies in aqueous media

Paper ID Volume ID Publish Year Pages File Format Full-Text
4229 216 2009 10 PDF Available
Title
Enantioselective whole-cell biotransformation of acetophenone to S-phenylethanol by Rhodotorula glutinis: Part I. Product formation kinetics and feeding strategies in aqueous media
Abstract

This work explores product-formation kinetics of the reduction of acetophenone to (S)-phenylethanol by Rhodotorula glutinis in aqueous media. Different feeding strategies were investigated to improve biotransformation productivity. An empirical kinetic model was formulated considering two different reaction periods: Phase-1, a very fast chemical-shock reaction in the first 5 min; Phase-2, a subsequent slower reaction which, depending on the product level, could be subinhibitory or inhibitory. The kinetics of the biotransformation metabolism were modulated by the levels of glucose and acetic acid in the bioreactor. In addition, two substrate feeding regimes were compared: intermittent and continuous. Although similar overall productivities were found, the intermittent method clearly holds greater potential for enhanced space-time yields as it takes advantage of the Phase-1 high reaction rates – additions of substrate pulses produce reaction rates of the same order of magnitude as those achieved in Phase-1. However, product accumulation to a “toxic limit” leads to the total cessation of biocatalytic activity, thus highlighting the importance of in-situ product removal during the biotransformation. Strategies for in-situ product removal are addressed in Part II of this study.

Keywords
Aph, acetophenone; FB-Int, fed-batch bioreactor with intermittent substrate addition; FB-Cont, fed-batch bioreactor with continuous substrate addition; FB-Cont-Glu, fed-batch bioreactor with continuous substrate and glucose addition; Grow-FB, growing-cell
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Enantioselective whole-cell biotransformation of acetophenone to S-phenylethanol by Rhodotorula glutinis: Part I. Product formation kinetics and feeding strategies in aqueous media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 1, 1 September 2009, Pages 44–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us